Background: Premature rupture of membrane (PROM) is a condition with spontaneous rupture of foetal membranes after 37 completed weeks but before onset of true labor pain. [1] PROM occurs in approximately 1-3% of all pregnancies and 30-40% causes of all preterm labor [2]. PROM mostly has variety of causes but many believe that intra uterine infection is one of the most important events of the cause.

Methods: Patient was asked to lie down on examination table in lithotomy position. Then perineal area and vagina is cleansed with sterile normal saline. Sim’s speculum is positioned in posterior vaginal wall and posterior lip of cervix was held by vulsellum forceps, then sterile cotton swab stick was introduced into the posterior fornix and two high vaginal swab was collected with all septic precautions.

Result: A total of 71 pregnant women with gestational age 37 weeks or more complicated by PROM along with 71 pregnant women with gestational age 37 weeks or more undergoing normal labor, who fits the inclusion criteria were evaluated during a period of 1 year attending the emergency room and labor room of OBGY Department in College of Medicine and J.N.M Hospital, Kalyani Nadia. 

Conclusion: Present study was conducted at the Department of Microbiology, College of Medicine & J.N.M Hospital, Kalyani (Nadia) W.B between June 2018 to June 2019 where high vaginal swabs from 71 patients with PROM and 71 from without-PROM were taken for bacteriological study. Among 71 samples from PROM cases 39 samples showed growth and 32 samples showed no growth. Among 71 samples of NON-PROM cases 54 samples showed growth and 17 samples showed no growth.

Keywords: PROM, Intrauterine Infection, Preterm Labor

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